Fig. 5

Single-cell characterization of ammonia death-related signatures in ccrcc. (A) UMAP (Uniform Manifold Approximation and Projection) mapping of major cell populations. (B) Violin chart compared AS of normal and ccRCC cells. (C, D) Heat maps showing the cell type-specific expression of ammonia metabolization-related genes in immune/stromal subsets. (E) The dot plots showed the differential expression of genes related to ammonia metabolism in each cell population. (F) Violin plot showed the distribution of BAX expression in different cell types. (G, H) Spatial UMAP projection map BAX representation localization in TME. (I) Stacked bar plots quantified the proportion of tumor cells with high/low BAX expression. (J) Bubble map analysis of ligand-receptor interactions between BAX + tumor cells and immunosuppressed populations. (K) Heat maps showing the correlation between ammonia metabolism-related cell clusters and BAX-associated immune subsets. (L) Network diagram summarizes the frequency and intensity of interactions between BAX + tumor cells and immunosuppressive components