Fig. 2

Profiles of cfDNA fragment size and end motifs. (A) Comparison of the fragment size distribution of different groups, which all peaked at 166 bp. Pre: fasting state, post1: 30 min after the meal, post2: 2 h after the meal; (B) Comparison of the differences in the proportion of fragment size ranges of 70–150 bp, 151–250 bp, and 251–400 bp between different groups. P values for paired Wilcoxon tests for paired linkage plots are provided; (C) Differentially enriched end motifs 30 min after meals compared with fasting state. (D) General scheme of NNLS analysis for the 4-mer end-motif profiles. (E) Results of end motif deconvolution analyses of plasma cfDNA from different groups; (F) Comparison of F-profile I, F-profile II, and F-profile III levels between different groups. P values for paired Wilcoxon tests for paired linkage plots are provided