Fig. 1

The graphic workflow and IHC staing of AQP1. (A) The graphic workflow of our study. The left half of the figure detailed the clinical cohort aspect of our study, which encompassed AQP1 staining and subsequent analysis of its correlation with patient survival outcomes. The right half provided a visual overview of our pan-cancer research, focusing on the analysis of prognostic relationships, tumor immunity, and genomic factors. Created with BioRender.com. (B) 24 staining results each from tumor and normal tissues were selected and displayed. The images in the top right corner were magnified 10 times, while those in the center were magnified 40 times, providing detailed visual insights. All tumor tissues had an IHC score of 0, whereas normal tissues included IHC scored12. IHC, Immunohistochemical