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Fig. 5 | Human Genomics

Fig. 5

From: rs2013278 in the multiple immunological-trait susceptibility locus CD28 regulates the production of non-functional splicing isoforms

Fig. 5

Binding of CD80 and CD86 with each CD28 splicing isoform product. a–c HeLa cells with transiently overexpressed GFP-conjugated CD28 and CD28i were incubated in the presence of His-tag conjugated CD86-Fc and PE-conjugated anti-His-Tag antibody. Dot plot for mock (a), CD28 (b), and CD28i (c). The horizontal and vertical axes indicate the expression of GFP-tagged CD28 isoforms and the bindings of His-tagged CD86-Fc, respectively. d, e In silico prediction of binding between CD80 and CD28 splicing isoforms. Although binding between CD80 and CD28 was predicted (d), that between CD80 and CD28i was not (e)

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Human Genomics

ISSN: 1479-7364