Fig. 4

ACE2 variants subcellular localization. HeLa cells were grown on coverslips and transiently co-transfected with Flag-tagged ACE2 WT or missense variants and GFP-tagged HRas (plasma membrane marker). 24 Hrs post transfection, cells were fixed and stained with anti-Flag and anti-Calnexin (ER marker) antibodies. Images were acquired using 100X magnification and manipulated by ImageJ. Scale bar = 50 μm